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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in several tumors, which leads to chromosome instability, and so may contribute to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually described. Two additional splice variants ended up noted but detailed nucleotide sequence facts just isn't nonetheless offered.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide typically has the epitope recognized through the antibody.
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This manual outlines even more achievable optimization of cellular and immunoassay parameters to be certain the very best final results are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to ensure the very best results are...
Antibodies bound to the blocking peptide now not bind to the epitope around the target protein. By evaluating the staining in the blocked antibody compared to the antibody on your own, one can see which staining is particular.
Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose users are characterized by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins show distinct expression and degradation patterns which contribute for the temporal coordination of each mitotic event. Cyclin E sorts a fancy with and functions for a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary and is also degraded as cells development through S stage.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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